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Direct enantiospecific HPLC bioanalysis of (R,S)‐atenolol on a chiral stationary phase
Author(s) -
Kofahl Birgit,
Henke Dorit,
Mutschler Ernst
Publication year - 1993
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530050614
Subject(s) - chemistry , enantiomer , chromatography , bioanalysis , atenolol , chiral stationary phase , high performance liquid chromatography , chiral column chromatography , urine , pharmacokinetics , silica gel , dichloromethane , pindolol , organic chemistry , pharmacology , solvent , receptor , medicine , biochemistry , blood pressure , radiology
The simultaneous determination of the enantiomers of the β 1 ‐selective adrenergic antagonist atenolol in human plasma and urine is described. After an alkaline preextraction atenolol is extracted from biological material at pH 12.3 using dichloromethane/propan‐2‐ol. The separation of the underivatized enantiomers is achieved by high‐performance liquid chromatography on a chiral stationary phase (Chiralcel OD, cellulose tris‐3, 5‐dimethylphenylcarbamate, coated on silica gel) with fluorimetric detection. (−)‐(S)‐Pindolol is used as an internal standard. The detection limits of 5 ng/ml enantiomer in plasma and 50 ng/ml enantiomer in urine are sufficient for pharmacokinetic studies after therapeutic doses. © 1993 Wiley‐Liss, Inc.