Premium
HPLC separation of the enantiomers of nicotine and nicotine‐like compounds
Author(s) -
Demetriou Demetrios,
Rustemeier Klaus,
Voncken Peter,
Schepers Georg
Publication year - 1993
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530050504
Subject(s) - chemistry , nornicotine , enantiomer , nicotine , chromatography , high performance liquid chromatography , chromatographic separation , alkaloid , organic chemistry , neuroscience , biology
A sensitive and reproducible HPLC method utilizing a commercially available chiral α 1 ‐acid glycoprotein (AGP) phase has been developed to separate and quantify the enantiomers of nicotine. The method is suitable for routine use as indicated by column life. The quantification of (R/S:0.05/99.95)‐nicotine or (R/S:99/1)‐nicotine was possible. In addition, the separation or at least partial separation of the enantiomers of nornicotine and nornicotine‐derived compounds was achieved. © 1993 Wiley‐Liss, Inc.