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Preparative separation of amino acid enantiomers as N ‐TFA, O ‐isopropyl derivatives by continuous countercurrent gas‐liquid chromatography
Author(s) -
Sato Katsuya,
Watabe Katsunori,
Ihara Toshihide,
Hobo Toshiyuki
Publication year - 1993
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530050411
Subject(s) - chemistry , enantiomer , countercurrent exchange , chromatography , diluent , isopropyl , countercurrent distribution , leucine , valine , amino acid , countercurrent chromatography , gas chromatography , organic chemistry , high performance liquid chromatography , biochemistry , physics , thermodynamics
Abstract Preparative separation of derivatives of amino acid enantiomers was carried out by a countercurrent gas–liquid chromatography (CCGLC) with chiral liquid phases, N ‐stearoyl‐ L ‐valine tert ‐butylamide and/or N ‐stearoyl‐ L ‐leucine tert ‐butylamide. In order to make effective use of these phases and also to lower the viscosity, Apiezon C was added as diluent. Through a repeated operation of a temperature gradient, purities more than 99% of leucine and α‐amino butyric acid derivatives were proved to be obtained. © 1993 Wiley‐Liss, Inc.