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Enantioselective determination of hydroxychloroquine and its major metabolites in urine and the observation of a reversal in the (+)/(−)‐hydroxychloroquine ratio
Author(s) -
Fieger Hiltrud,
Iredale Joanna,
Wainer Irving W.
Publication year - 1993
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530050205
Subject(s) - chemistry , hydroxychloroquine , enantiomer , urine , chromatography , enantioselective synthesis , high performance liquid chromatography , pharmacology , stereochemistry , medicine , organic chemistry , biochemistry , catalysis , disease , covid-19 , infectious disease (medical specialty)
Abstract A sequential achiral‐chiral high‐performance liquid chromatographic system has been developed for the quantitation in urine of the enantiomers of hydroxychloroquine (HCQ), and of its 3 major metabolites, desethylhydroxychloroquine (DHCQ), desethylchloroquine (DCQ), and bisdesethylchloroquine (BDCQ). HCQ and its metabolites were separated and quantified on a cyano‐bonded phase, and the enantiomeric ratios were determined using a Chiral‐AGP chiral stationary phase. The assay validation and application of this method to a preliminary study in a human volunteer are presented. In this subject, the initial 0‐4 h urine contained the 2 HCQ enantiomers in a ratio of (+)‐HCQ:(−)‐HCQ of 3:2; by the 2,064 h of the study, this ratio had reversed to (+)‐HCQ:(−)‐HCQ of 3:7. © 1993 Wiley‐Liss, Inc.