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Determination of the enantiomeric purity of (−−) 2‐(N‐propyl‐n‐2‐thienylethylamino)‐5‐hydroxytetralin (n‐0923) by chiral stationary phase HPLC
Author(s) -
Witte Dirk T.,
Franke JanPiet,
Swart Pieter J.,
De Zeeuw Rokus A.
Publication year - 1992
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530040113
Subject(s) - chemistry , enantiomer , calibration curve , chromatography , impurity , high performance liquid chromatography , absorbance , reproducibility , enantiomeric excess , phase (matter) , analytical chemistry (journal) , chiral stationary phase , detection limit , stereochemistry , organic chemistry , enantioselective synthesis , catalysis
The determination of the enantiomeric impurity, i.e., the percentage of (+) N−0437 (= N−0924) in several batches of (−−) N‐0437 (= N‐0923) by chiral HPLC is described. Enantiomeric impurities were calculated based on the peak areas of the two baseline separated enantiomers in the chromatogram. The enantiomeric impurities found in different batches ranged from 0.02% to 0.11%. Calibration curves of the two isomers of N‐0437 (Fig. 1,) were made twice to study the reproducibility and linearity of the method. The absorbance ratio, N‐0923/N‐0924, was found to be 1.02 with a relative standard deviation (RSD) of 9% over the whole concentration range used for the calibration curves.