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Circular dichroism detection in HPLC
Author(s) -
Salvadori Piero,
Bertucci Carlo,
Rosini Carlo
Publication year - 1991
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530030424
Subject(s) - chemistry , circular dichroism , enantiomer , elution , chromatography , high performance liquid chromatography , molecule , optically active , resolution (logic) , analytical chemistry (journal) , stereochemistry , organic chemistry , artificial intelligence , computer science
A circular dichroism‐based detection system presents several advantages in the HPLC analysis of chiral compounds because of the selective monitoring of optically active molecules. Its use allows reliable determination of enantiomeric excesses and elution order. To this end, the application of empirical, semiempirical, and nonempirical methods to get stereochemical information from the CD signal is reported. Furthermore, recording the CD spectra on line and evaluation of the dissymetry factor make the CD detection very powerful in characterizing the stereochemistry of chiral eluates.