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The circular dichroism of tumor necrosis factor‐α: Measurement into the vacuum UV and analysis for secondary structure
Author(s) -
RiazanceLawrence Jeannine H.,
Toumadje Arazdordi,
Johnson W. Curtis
Publication year - 1991
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530030407
Subject(s) - chemistry , circular dichroism , protein secondary structure , diffraction , beta (programming language) , crystallography , optics , biochemistry , physics , programming language , computer science
The circular dichroism (CD) spectrum of tumor necrosis factor‐α has been measured into the vacuum UV to 168 nm. Analysis of the CD for secondary structure is in good agreement with X‐ray diffraction results, but the analysis is somewhat unstable. Adding the CD of this protein together with its X‐ray determined secondary structure to the basis set should improve subsequent analyses of CD spectra for other all‐β proteins.