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A method for the quantitative enantioselective HPLC analysis of ethambutol and its stereoisomers
Author(s) -
Blessington B.,
Beiraghi A.
Publication year - 1991
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530030211
Subject(s) - chemistry , chromatography , enantioselective synthesis , high performance liquid chromatography , chiral stationary phase , quantitative analysis (chemistry) , hexane , organic chemistry , catalysis
A rapid, quantitative, enantioselective HPLC method, using N , N ′‐dibenzylethylenediamine as internal standard, for the analysis of the three stereoisomers of the antituberculosis drug ethambutol and 2‐aminobutan‐1‐ol is described. The derivatisation and chromatographic procedures and their reproducibility, durability, and sensitivity are discussed. Application to assays of raw material, tablet content, and synthetic reactions are illustrated. The method involves perbenzoylation, in pyridine, followed by direct HPLC on a “Pirkle covalent bonded column” containing a 3,5‐dinitrobenzoyl‐ D ‐phenylglycine as chiral stationary phase (CSP) using hexane/IPA as mobile phase and detection at 230 nm.