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Direct measurement of enantiomeric ratios of enzymatic resolution by chiral high‐performance liquid chromatography
Author(s) -
Wu ShihHsiung,
Lai SuYuan,
Lin ShuLing,
Chu FeiYa,
Wang KungTsung
Publication year - 1991
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530030111
Subject(s) - chemistry , enantiomer , propionate , enantiomeric excess , chromatography , resolution (logic) , substrate (aquarium) , chiral derivatizing agent , organic chemistry , chiral column chromatography , enantioselective synthesis , catalysis , oceanography , artificial intelligence , geology , computer science
(R)‐ and (S)‐Methyl 2‐(phenoxy)propionate and their acids could be separated simultaneously by a Chiralcel OD or OK column, while (R)‐ and (S)‐methyl 2‐(4‐chlorophenoxy)propionate and their acids were separated concurrently only by an OK column. This is a novel and facile way to measure the enantiomeric excesses of the remaining substrate and product in the reaction of enzymatic resolution; enantiomeric ratios could then be calculated.