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An HPLC procedure for the enantiomeric purity of an optically active cytosine analogue using ligand exchange
Author(s) -
Pack Edward J.,
Bleiberg Bonnie,
Rosenberg Ira E.
Publication year - 1990
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530020414
Subject(s) - chemistry , enantiomer , cytosine , ligand (biochemistry) , high performance liquid chromatography , phenylalanine , optically active , chiral column chromatography , chromatography , stereochemistry , combinatorial chemistry , organic chemistry , dna , amino acid , biochemistry , receptor
An acylonucleotide analogue, 1‐[3‐hydroxy‐2‐(phosphonyl‐methoxy)propyl]cytosine (HPMPC), has shown activity against herpes simplex Type I and Type II viruses. An HPLC separation of the R‐ and S‐enantiomers of HPMPC by ligand exchange using a mobile phase containing phenylalanine as the chiral modifier and copper(II) as the metal ion is reported.