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Enantioselective binding of mephobarbital to plasma proteins
Author(s) -
O'shea Nerida J.,
Hooper Wayne D.
Publication year - 1990
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530020411
Subject(s) - chemistry , enantiomer , enantioselective synthesis , plasma protein binding , albumin , blood proteins , in vitro , blood plasma , binding site , serum albumin , human serum albumin , pharmacokinetics , stereochemistry , chromatography , biochemistry , medicine , catalysis
The enantioselective protein binding of mephobarbital (MPB) was investigated in human plasma and human serum albumin solutions by equilibrium dialysis. A small but statistically significant difference was observed in the in vitro plasma protein binding of the enantiomers; (S)‐MPB was ∼59% bound and (R)‐MPB ∼67% bound. The binding to albumin [(S)‐MPB: ∼29% bound, and (R)‐MPB: ∼41% bound] was less than to plasma proteins but showed somewhat greater enantioselectivity, suggesting that albumin binding is a major source of the enantioselectivity in plasma. The effects of MPB concentration, of varying enantiomeric concentration ratio, and of phenobarbital on the enantioselective binding of MPB were studied. The effect of age was also investigated by measuring the binding in plasma from 8 young (18–25 yr) and 8 elderly (>60 yr) male subjects who took single doses of MPB. The results were in close agreement with the in vitro binding data, and the binding of both enantiomers was marginally but significantly lower in the young compared with the elderly subjects. These differences in binding were consistent with previously observed pharmacokinetic differences between the two subject groups.