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Stereochemical features of 1,4‐benzodiazepin‐2‐ones bound to human serum albumin: Difference CD and UV studies
Author(s) -
Bertucci Carlo,
Domenici Enrico,
Salvadori Piero
Publication year - 1990
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530020308
Subject(s) - chemistry , circular dichroism , human serum albumin , absorption (acoustics) , stereochemistry , serum albumin , albumin , diazepam , chromatography , biochemistry , pharmacology , medicine , physics , acoustics
The stereochemistry of an achiral (Diazepam) and two chiral (3‐methyl and 3‐succinyloxy substituted) 1,4‐benzodiazepin‐2‐ones interacting with human serum albumin (HSA) has been investigated by making use of difference absorption (UV) and circular dichroism (CD) spectroscopies. Evidence is obtained for a higher affinity with HSA for one of the two possible conformations of the seven‐membered benzodiazepine ring. The red shift revealed by the absorption difference spectrum between the free and the bound drug accounts for the CD difference spectra observed.