Premium
Stereoselective effect of warfarin and bilirubin on the binding of 5‐( o ‐chlorophenyl)‐1,3‐dihydro‐3‐methyl‐7‐nitro‐2 H ‐1,4‐ benzodiazin‐2‐one enantiomers to human serum albumin
Author(s) -
Fitos Ilona,
Visy Julia,
Magyar Anna,
Simonyi MiklóS,
Kajtár Judit
Publication year - 1990
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530020307
Subject(s) - chemistry , bilirubin , stereoselectivity , enantiomer , oxazepam , benzodiazepine , human serum albumin , stereochemistry , circular dichroism , warfarin , chromatography , biochemistry , receptor , catalysis , medicine , gastroenterology , cardiology , atrial fibrillation
The binding of the title benzodiazepine enantiomers and its modulation by warfarin and bilirubin were studied by chromatography on human serum albumin (HSA) immobilized on Sepharose 4B, and also by a combination of ultrafiltration and circular dichroism (UF‐CD) methods. In the absence of warfarin and bilirubin the binding of the benzodiazepine was not stereoselective. (S)‐Benzodiazepine and (S)‐warfarin mutually increased the binding of each other, while the binding of (R)‐benzodiazepine was preferentially enhanced on HSA saturated with bilirubin.