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Improved performance of the second generation α 1 ‐AGP columns: Applications to the routine assay of plasma levels of alfuzosin hydrochloride
Author(s) -
Krstulovic A. M.,
Vende J. L.
Publication year - 1989
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530010311
Subject(s) - chemistry , alfuzosin , chromatography , enantiomer , hydrochloride , extraction (chemistry) , pharmacokinetics , stereochemistry , pharmacology , organic chemistry , prostate , lower urinary tract symptoms , medicine , cancer
Described is a direct enantioselective separation of the enantiomers of alfuzosin hydrochloride on the second generation α 1 ‐AGP column which offers improved efficiency, shorter analysis, and improved stability with respect to the first generation columns. The method has been applied to the analysis of drug substance in rat plasma. This highly efficient extraction method and the use of fluorimetric detection result in selective and sensitive determination of the enantiomers. The analytical validation parameters demonstrate the applicability of this method to pharmacokinetic and metabolic studies.