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Separation and quantitation of (R)‐ and (S)‐atenolol in human plasma and urine using an α 1 ‐AGP column
Author(s) -
Enquist Märit,
Hermansson Jörgen
Publication year - 1989
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.530010306
Subject(s) - chemistry , chromatography , atenolol , urine , human plasma , column (typography) , plasma , separation (statistics) , biochemistry , medicine , blood pressure , physics , structural engineering , connection (principal bundle) , quantum mechanics , machine learning , computer science , engineering
A method for the determination of (R)‐ and (S)‐atenolol in human plasma and urine is described. The enantiomers of atenolol are extracted into dichloromethane containing 3% heptafluorobutanol followed by acetylation with acetic anhydride at 60°C for 2 h. The acetylated enantiomers were separated on a chiral α 1 ‐AGP column. Quantitation was performed using fluorescence detection. A phosphate buffer pH 7.1 (0.01 M phosphate) containing 0.25% (v/v) acetonitrile was used as mobile phase. The described procedure allows the detection of less than 6 ng of each enantiomer in 1 ml plasma. The relative standard deviation is 4.4% at 30 ng/ml of each enantiomer in plasma. The plasma concentration of (R)‐ and (S)‐ atenolol did not differ significantly in two subjects who received a single tablet of racemic atenolol. The R/S ratio of atenolol in urine was ∼ 1.