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Bioanalytical chiral chromatographic technique and docking studies for enantioselective separation of meclizine hydrochloride: Application to pharmacokinetic study in rabbits
Author(s) -
Raikar Prachi,
Gurupadayya Bannimath,
Mandal Subhankar P.,
Narhari Rishitha,
Subramanyam Sripuram,
Srinivasu Gunnam,
Rajan Surulivel,
Saikumar Matcha,
Koganti Sairam
Publication year - 2020
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.23241
Subject(s) - chemistry , enantiomer , enantioselective synthesis , chromatography , hydrochloride , bioanalysis , ammonium bicarbonate , elution , docking (animal) , combinatorial chemistry , organic chemistry , catalysis , medicine , nursing , raw material
Enantiomeric resolution and molecular docking studies of meclizine hydrochloride on polysaccharide‐based chiral stationary phase comprising cellulose tris(4‐methylbenzoate) chiral selector (150 × 4.6 mm, 3.0 μm) were presented. The mobile phase used was acetonitrile:10mM ammonium bicarbonate (95:05, v/v). The developed technique was used to perform the enantioselective assay of meclizine hydrochloride in its marketed formulation. The elution order of meclizine hydrochloride enantiomers was determined by docking studies. Target compound was extracted from rabbit plasma using protein precipitation technique, followed by development of bioanalytical chiral separation method using the same matrix. Application of the method to determine pharmacokinetic parameters of meclizine hydrochloride enantiomers was performed using Phoenix WinNonlin 8.1 software. The results demonstrated stereoselective disposition of meclizine hydrochloride enantiomers in rabbits.