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Chiral separation of underivatized amino acids in supercritical fluid chromatography with chiral crown ether derived column
Author(s) -
Miller Larry,
Yue Lei
Publication year - 2020
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.23204
Subject(s) - chemistry , chromatography , supercritical fluid chromatography , enantiomer , crown ether , amino acid , elution , ether , chiral column chromatography , resolution (logic) , supercritical fluid , high performance liquid chromatography , organic chemistry , ion , biochemistry , artificial intelligence , computer science
The supercritical fluid chromatographic separation of underivatized amino acids was explored using immobilized chiral crown ether column CROWNPAK CR‐I (+) and mass spectrometric detection. The type of modifier, acidic additives, and the role of water were investigated. Enantioseparation was achieved for all 18 amino acids investigated with short retention times (less than 3 minutes) and average resolution of greater than 5.0. Analysis of enantiomerically pure standards demonstrated the D enantiomer eluted first for all amino acids using a CROWNPAK CR‐I (+) column.