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Comparison of polysaccharide‐based and protein‐based chiral liquid chromatography columns for enantioseparation of drugs
Author(s) -
Fu Yandi,
Borrull Francesc,
Fontanals Núria,
Marcé Rosa Maria
Publication year - 2020
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.23198
Subject(s) - chemistry , diethylamine , chromatography , elution , acetonitrile , ammonium acetate , formic acid , methanol , ammonium hydroxide , cellulose , ammonium , high performance liquid chromatography , organic chemistry
Two different columns—Lux Cellulose‐1 and Chiralpak CBH—were evaluated for their chiral recognition abilities for eight drugs comprising three β‐blockers, one antacid, and four cathinones in polar‐organic elution mode and reversed‐phase elution mode, respectively. The factors that affected the enantioseparation were tested and optimized to develop a suitable chiral separation method whose LC conditions are compatible with MS detection. In polar‐organic elution mode with the Lux Cellulose‐1 column, methanol and acetonitrile were tested as the main components of the mobile phase. In addition, the effects of adding isopropanol as organic modifier, acidic additives (formic acid), and basic additives (diethylamine) were evaluated. In reversed‐phase elution mode with the Chiralpak CBH column, the effect of type and concentration of organic modifier (isopropanol, acetonitrile, and methanol), the mobile phase pH (6.4 and 5.0), and buffer concentration (1mM‐20mM ammonium acetate) were evaluated. The best enantioseparation was achieved with the Chiralpak CBH column with a mobile phase composed of 5mM ammonium acetate aqueous (pH = 6.4)/methanol (95/5, v/v) at a flow rate of 0.1 mL/min and a temperature of 30°C. Under these conditions, six of eight chiral drugs were baseline separated.