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Fast liquid chromatography for racemic atenolol acetate separation— T he analytical protocol
Author(s) -
Agustian Joni,
Kamaruddin Azlina Harun,
AboulEnein Hassan Y.
Publication year - 2017
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.22769
Subject(s) - chemistry , atenolol , chromatography , enantiomer , resolution (logic) , amine gas treating , phase (matter) , chiral resolution , analytical chemistry (journal) , organic chemistry , medicine , artificial intelligence , computer science , blood pressure , radiology
Kinetic resolution of ( R , S )‐atenolol is a faster strategy to produce ( S )‐atenolol. Since this racemate is a less soluble compound, resolution of its ester offers high concentrations in the process. A good analytical method is required to observe the enantiomer concentrations. This paper described application of ultra‐fast liquid chromatography on the atenolol ester separation using different resolution media and analytical procedures. Chiralcel OD column resolved the ester. The chromatograms indicated different characteristics of the process. The enantiomers could be recognized by the column in less than 1 (one) hour. Symmetrical peaks were obtained, but several procedures produced peaks with wide bases and slanted baselines. Efficient enantioresolution was obtained at high mobile phase flow rate, decreased concentration of amine‐type modifier, but increased alcohol content in the mobile phase. High UV detection wavelength was required. At 1.0 mL/min, the (90/10/0.5) composition resulted α = 1.46 and R S = 0.9998 that were good separation.