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Effect of Different Z‐Inducers on the Stabilization of Z Portion in BZ‐DNA Sequence: Correlation Between Experimental and Simulation Data
Author(s) -
Pietropaolo Adriana,
D'urso Alessandro,
Purrello Roberto,
Berova Nina
Publication year - 2015
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.22502
Subject(s) - chemistry , sequence (biology) , dna , inducer , correlation , stereochemistry , biophysics , biochemistry , gene , geometry , mathematics , biology
In this study we show the outstanding agreement between simulation and experimental data concerning the efficient stabilization effect by NaCl of Z conformation. We demonstrate by circular dichroism (CD) experiments that Na + not only is able to induce a B to Z form transition in a short (GC) 3 alternated portion of a sequence having 17 basis, but also is the best stabilizer in comparison with other Z inducers used (spermine and NiCl 2 ). This result was confirmed by free energy calculations. Chirality 27:773–778, 2015 . © 2015 Wiley Periodicals, Inc.