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Electrically Assisted Liquid‐Phase Microextraction Combined With Capillary Electrophoresis for Quantification of Propranolol Enantiomers in Human Body Fluids
Author(s) -
Tabani Hadi,
Fakhari Ali Reza,
Shahsavani Abolfath,
Gharari Alibabaou Hossein
Publication year - 2014
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.22308
Subject(s) - chemistry , capillary electrophoresis , enantiomer , chromatography , solid phase microextraction , propranolol , liquid liquid , capillary action , electrophoresis , phase (matter) , mass spectrometry , organic chemistry , gas chromatography–mass spectrometry , medicine , materials science , composite material
In this study, electromembrane extraction (EME) combined with cyclodextrin (CD)‐modified capillary electrophoresis (CE) was applied for the extraction, separation, and quantification of propranolol (PRO) enantiomers from biological samples. The PRO enantiomers were extracted from aqueous donor solutions, through a supported liquid membrane (SLM) consisting of 2‐nitrophenyl octyl ether (NPOE) impregnated on the wall of the hollow fiber , and into a 20‐μL acidic aqueous acceptor solution into the lumen of hollow fiber . Important parameters affecting EME efficiency such as extraction voltage, extraction time, pH of the donor and acceptor solutions were optimized using a Box‐Behnken design (BBD). Then, under these optimized conditions, the acceptor solution was analyzed using an optimized CD‐modified CE. Several types of CD were evaluated and best results were obtained using a fused‐silica capillary with ammonium acetate (80 mM, pH 2.5) containing 8 mM hydroxypropyl‐β‐CD as a chiral selector, applied voltage of 18 kV, and temperature of 20°C. The relative recoveries were obtained in the range of 78–95%. Finally, the performance of the present method was evaluated for the extraction and determination of PRO enantiomers in real biological samples. Chirality 26:260–267, 2014. © 2014 Wiley Periodicals, Inc.