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Enantioselective Surface Plasmon Resonance Sensor Based on C 60 Fullerene‐Glutathione Self‐Assembled Monolayer (SAM)
Author(s) -
Stefanvan Staden RalucaIoana
Publication year - 2014
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.22281
Subject(s) - chemistry , surface plasmon resonance , monolayer , chirality (physics) , fullerene , dissociation constant , enantioselective synthesis , histidine , binding constant , resonance (particle physics) , glutathione , biosensor , photochemistry , analytical chemistry (journal) , organic chemistry , nanotechnology , binding site , nanoparticle , materials science , catalysis , enzyme , biochemistry , chiral symmetry breaking , physics , receptor , particle physics , quantum mechanics , quark , nambu–jona lasinio model
A novel enantioselective surface plasmon resonance (SPR) sensor based on a self‐assembled monolayer of C 60 fullerene as the chiral selector is proposed. A binding assay, apparent affinity constant, and apparent dissociation binding constant were used to analyze and study the enantioselectivity of C 60 fullerene‐glutathione film for L‐histidine, which was chosen as the model analyte. The apparent affinity constant for the complex formed by L‐histidine with C 60 fullerene‐glutathione film was 5.2 x 10 9 M ‐1 . The proposed SPR sensor can be used for the assay of L‐histidine in the 10 ‐10 – 10 ‐7 mol/L concentration range. Chirality 26:129–131, 2014. © 2014 Wiley Periodicals, Inc.