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Electrochromatographic Enantioseparation of Amino Acids Using Polybutylmethacrylate‐based Chiral Monolithic Column by Capillary Electrochromatography
Author(s) -
Aydogan Cemil,
Denizli Adil
Publication year - 2012
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.22065
Subject(s) - chemistry , capillary electrochromatography , chromatography , enantiomer , monolithic hplc column , electrochromatography , amino acid , high performance liquid chromatography , phosphate , capillary electrophoresis , organic chemistry , biochemistry
This article describes the development of a polybutylmethacrylate‐based monolithic capillary column as a chiral stationary phase. The chiral monolithic column was prepared by polymerization of butyl methacrylate (BMA), ethylene dimethacrylate (EDMA), and N ‐methacryloyl‐ l ‐glutamic acid (MAGA) in the presence of porogens. The porogen mixture included N , N ‐dimethyl formamide and phosphate buffer. MAGA was used as a chiral selector. The effect of MAGA content was investigated on electrochromatographic enantioseparation of d,l ‐histidine, d,l ‐tyrosine, d,l ‐phenyl alanine, and d,l ‐glutamic acid. The effect of acetonitrile (ACN) content in mobile phase on electro‐osmotic flow was also investigated. It was demonstrated that the poly(BMA‐EDMA‐MAGA) monolithic chiral column can be used for the electrochromatographic enantioseparation of amino acids by capillary electrochromatography (CEC). The mobile phase was ACN/10 mM phosphate buffer (45:55%) adjusted to pH 2.7. It was observed that l ‐enantiomers of the amino acids migrated before d ‐enantiomers. The separation mechanism of electrochromatographic enantioseparation of amino acids in CEC is discussed. Chirality 24:606–609, 2012 . © 2012 Wiley Periodicals, Inc.