Premium
Simultaneous enantioselective separation of polychlorinated biphenyls and their methyl sulfone metabolites by heart‐cut MDGC: Determination of enantiomeric fractions in fish oils and cow liver samples
Author(s) -
PérezFernández Virginia,
CastroPuyana María,
González María José,
Marina María Luisa,
García María Ángeles,
Gómara Belén
Publication year - 2012
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.22062
Subject(s) - chemistry , enantioselective synthesis , chromatography , enantiomer , elution , resolution (logic) , gas chromatography , atropisomer , organic chemistry , catalysis , artificial intelligence , computer science
The potential of three capillary columns based on β‐cyclodextrin (i.e., Chirasil‐Dex, BGB‐172, and BGB‐176SE) has been studied for the simultaneous enantiomeric separation of polychlorinated biphenyls (PCBs) and methylsulfonyl metabolites of PCBs (MeSO 2 ‐PCBs) employing a heart‐cut multidimensional gas chromatographic system (heart‐cut MDGC). Among the columns studied, the BGB‐176SE capillary column provided the best results, allowing the simultaneous enantioselective resolution of six MeSO 2 ‐PCBs and six chiral PCBs; the Chirasil‐Dex column did not resolve any of the studied MeSO 2 ‐PCBs; and a poor resolution was obtained for three MeSO 2 ‐PCBs when the BGB‐172 column was employed. The developed method was successfully applied to two fish oil and one cow liver samples commercially available, which showed different enantioselective pattern. PCBs 91 and 176 presented a clear enrichment of the second eluted atropisomer in codfish oil, whereas in fish oil sample, slight enrichment of the first eluted atropisomer of CB45 and the second eluted atropisomer of CB136 were observed. Chirality 24:577–583, 2012 . © 2012 Wiley Periodicals, Inc.