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Chromatographic comparison of atenolol separation in reaction media on cellulose tris‐(3,5‐dimethylphenylcarbamate) chiral stationary phase using ultra fast liquid chromatography
Author(s) -
Agustian Joni,
Kamaruddin Azlina Harun,
AboulEnein Hassan Y.
Publication year - 2012
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.22019
Subject(s) - atenolol , chemistry , chromatography , enantiomer , resolution (logic) , chirality (physics) , phase (matter) , cellulose , chiral resolution , amine gas treating , solvent , analytical chemistry (journal) , organic chemistry , medicine , chiral symmetry breaking , physics , quantum mechanics , artificial intelligence , computer science , blood pressure , nambu–jona lasinio model , radiology , quark
Because chiral liquid chromatography (LC) could become a powerful tool to estimate racemic atenolol quantity, excellent enantiomeric separation should be produced during data acquisition for satisfactory observation of atenolol concentrations throughout the racemic resolution processes. Selection of chiral LC column and analytical protocol that fulfill demands of the ultra fast LC analysis is essential. This article describes the characteristics of atenolol chromatographic separation that resulted from different resolution media and analytical protocols with the use of a Chiralcel® OD column. The chromatograms showed quite different characteristics of the separation process. The single enantiomer and racemic atenolol could be recognized by the Chiralcel® OD column in less than 20 min. Symmetrical peaks were obtained; however, several protocols produced peaks with wide bases and slanted baselines. Observations showed that efficient enantioresolution of racemic atenolol was obtained at slow mobile phase flow rate, decreased concentration of amine‐type modifier but increased alcohol content in mobile phase and highest ultraviolet detection wavelength were required. The optimal ultra fast LC protocol enables to reduce and eliminate the peaks of either the atenolol solvent or the buffers and provided the highest peak intensities of both atenolol enantiomers. Chirality 24:356–367, 2012 . © 2012 Wiley Periodicals, Inc.