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Dichrometer Errors Resulting from Large Signals or Improper Modulator Phasing
Author(s) -
Sutherland John C.
Publication year - 2012
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.22006
Subject(s) - dichroic glass , linear dichroism , chemistry , circular dichroism , dichroism , fluorescence anisotropy , vibrational circular dichroism , absorbance , anisotropy , optics , magnetic circular dichroism , polarization (electrochemistry) , signal (programming language) , fluorescence , physics , crystallography , programming language , chromatography , astronomy , computer science , spectral line
ABSTRACT A single‐beam spectrometer equipped with a photoelastic modulator can be configured to measure a number of different parameters useful in characterizing chemical and biochemical materials including natural and magnetic circular dichroism, linear dichroism, natural and magnetic fluorescence‐detected circular dichroism, and fluorescence polarization anisotropy as well as total absorption and fluorescence. The derivations of the mathematical expressions used to extract these parameters from ultraviolet, visible, and near‐infrared light‐induced electronic signals in a dichrometer assume that the dichroic signals are sufficiently small that certain mathematical approximations will not introduce significant errors. This article quantifies errors resulting from these assumptions as a function of the magnitude of the dichroic signals. In the case of linear dichroism, improper modulator programming can result in errors greater than those resulting from the assumption of small signal size, whereas for fluorescence polarization anisotropy, improper modulator phase alone gives incorrect results. Modulator phase can also impact the values of total absorbance recorded simultaneously with linear dichroism and total fluorescence. Chirality 24:706–717, 2012 . © 2012 Wiley Periodicals, Inc. †