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Considerations of Noise and Measurement Reproducibility of Circular Dichroism Measurements Using Na[Co III (EDDS)]
Author(s) -
Chmel Nikola Paul,
Scott Peter,
Rodger Alison
Publication year - 2012
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.22005
Subject(s) - chemistry , edds , context (archaeology) , calibration , reproducibility , circular dichroism , chirality (physics) , standardization , analytical chemistry (journal) , noise (video) , enantiomer , spectroscopy , crystallography , stereochemistry , chromatography , environmental chemistry , physics , artificial intelligence , computer science , paleontology , chiral symmetry breaking , quantum mechanics , image (mathematics) , biology , quark , operating system , phytoremediation , heavy metals , nambu–jona lasinio model
Although circular dichroism (CD) spectroscopy has become ubiquitous in biological, chemical, and pharmacological laboratories, several issues with instrument calibration and standardization, measurements quality, and reproducibility still remain. In the context of validating and developing a wide‐wavelength‐range stable standard for CD that is available in both enantiomeric forms, ( R,R )‐ and ( S,S )‐Na[Co III (EDDS)] (where EDDS is ethylenediaminedisuccinato), we have become aware of sources of error in CD spectroscopy in addition to instrument calibration and user issues. This article summarizes those errors and suggests procedures for minimizing them by averaging over sufficient independent data accumulations with appropriate parameters. Chirality 24:699–705, 2012 . © 2012 Wiley Periodicals, Inc.