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HPLC–CD selectivity assay for alcohol dehydrogenases
Author(s) -
Hamzic Melissa,
Pietruszka Jörg,
Sandkuhl Diana
Publication year - 2011
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.21025
Subject(s) - chemistry , selectivity , enantioselective synthesis , circular dichroism , alcohol , chirality (physics) , alcohol dehydrogenase , high performance liquid chromatography , chromatography , trimethylsilyl , combinatorial chemistry , organic chemistry , catalysis , stereochemistry , chiral symmetry breaking , physics , quantum mechanics , nambu–jona lasinio model , quark
Enantioselective reductions are a key to successful target‐oriented syntheses. Finding the most suitable conditions is often a tedious work that is especially hampered by the time‐consuming analytical investigation. A possible solution is the combined use of high‐performance liquid chromatography and circular dichroism to find a suitable system for providing enantiomerically pure alcohols. This investigation led to an efficient protocol for the alcohol dehydrogenase‐catalyzed reduction of 1‐phenyl‐2‐propyn‐3‐trimethylsilyl‐1‐on ( 1 ). Chirality, 2011. © 2011Wiley Periodicals, Inc.