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Experimental test of absorption flattening correction for circular dichroism of particle suspensions
Author(s) -
Gerdova Anna,
Kelly Sharon M.,
Halling Peter
Publication year - 2011
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20973
Subject(s) - chemistry , absorbance , flattening , circular dichroism , scattering , volume fraction , particle (ecology) , absorption (acoustics) , analytical chemistry (journal) , chirality (physics) , suspension (topology) , light scattering , ethylenediamine , molecular physics , chromatography , crystallography , optics , physics , inorganic chemistry , oceanography , chiral symmetry breaking , mathematics , quantum mechanics , astronomy , quark , homotopy , pure mathematics , nambu–jona lasinio model , geology
There are well established theoretical models for correction for absorption flattening of circular dichroism (CD) measurements on particle suspensions. However, these have not been directly tested experimentally. We describe a test system with the chiral tris(ethylenediamine)Co(III) complex dissolved in water trapped inside sephadex particles, suspended in 1‐butanol. Independent measurements of particle size distribution , volume fraction, and the absorbance of the suspension are used to calculate the required CD correction. The corrected CD signal is found to agree rather well with that for the same amount of Co‐complex dispersed uniformly throughout the sample cell. This holds for different particle volume fractions and Co‐complex concentrations inside the particles. The correction seems to work despite a substantial scattering contribution to the absorbance, which is not considered in the theoretical models. Chirality, 2011. © 2011 Wiley‐Liss, Inc.