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Determination of catechin diastereomers from the leaves of Byrsonima species using chiral HPLC‐PAD‐CD
Author(s) -
Rinaldo Daniel,
Batista João M.,
Rodrigues Juliana,
Benfatti Ana C.,
Rodrigues Clenilson M.,
Dos Santos Lourdes C.,
Furlan Maysa,
Vilegas Wagner
Publication year - 2010
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20824
Subject(s) - chemistry , catechin , diastereomer , high performance liquid chromatography , chromatography , chirality (physics) , stereoselectivity , polyphenol , stereochemistry , organic chemistry , catalysis , nambu–jona lasinio model , chiral symmetry breaking , physics , quantum mechanics , antioxidant , quark
When catechins are found in plant extracts, they are almost always identified as catechin and/or epicatechin probably due to stereoselectivity of the enzymes involved in the biosynthesis of these substances. However, the lack of reports regarding to ent ‐catechin as well as ent ‐epicatechin does not necessarily mean that these compounds have not been produced. In fact, most of the previous reports used chromatographic conditions not suitable for such separation. This article describes a simple and reliable analytical HPLC‐PAD‐CD method for simultaneous determination of catechin diastereomers both in infusions and extracts from the leaves of Byrsonima species. The direct separation of catechin, ent ‐catechin, epicatechin, and ent ‐epicatechin was obtained in normal phase by HPLC‐PAD‐CD using Chiralcel OD‐H as chiral stationary phase and n ‐hexane/ethanol with 0.1% of TFA as mobile phase. Chirality, 2010. © 2010 Wiley‐Liss, Inc.