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Comparison of separation performances of amylose‐ and cellulose‐based stationary phases in the high‐performance liquid chromatographic enantioseparation of stereoisomers of β‐lactams
Author(s) -
Pataj Zoltán,
Ilisz István,
Berkecz Róbert,
Forró Enikő,
Fülöp Ferenc,
Péter Antal
Publication year - 2010
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20714
Subject(s) - chemistry , chromatography , enantiomer , elution , cellulose , carbamate , resolution (logic) , selectivity , amylose , chirality (physics) , high performance liquid chromatography , chiral column chromatography , organic chemistry , chiral symmetry breaking , physics , quantum mechanics , artificial intelligence , starch , computer science , nambu–jona lasinio model , quark , catalysis
High‐performance liquid chromatographic methods were developed for the separation of the enantiomers of 19 β‐lactams. The direct separations were performed on chiral stationary phases containing either amylose‐ tris ‐3,5‐dimethylphenyl carbamate, (Kromasil® AmyCoat™ column) or cellulose‐ tris ‐3,5‐dimethylphenyl carbamate, (Kromasil® CelluCoat™ column) as chiral selector. The different methods were compared in systematic chromatographic examinations. The separations were carried out with good selectivity and resolution. The AmyCoat™ and CelluCoat™ columns appear to be highly complementary. The best separations of bi‐ and tricyclic β‐lactam stereoisomers were obtained with the AmyCoat™ column, whereas the 4‐aryl‐substituted β‐lactams were better separated on the CelluCoat™ column. The elution sequence was determined in all cases; no general rule could be established. Chirality 2010. © 2009 Wiley‐Liss, Inc.