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Chiral separation performance of micrometer‐sized monodispersed silica spheres with high protein loading
Author(s) -
Zhai Zheng,
Chen Yang,
Wang YuJun,
Luo GuangSheng
Publication year - 2009
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20677
Subject(s) - chemistry , spheres , micrometer , separation (statistics) , chemical engineering , nanotechnology , chromatography , aerospace engineering , optics , physics , materials science , engineering , machine learning , computer science
Micrometer‐sized monodispersed silica spheres (∼360 μm) with high loading of bovine serum albumin (BSA) (∼450 mg/g) were prepared as an adsorbent for large‐scale chiral separation. The new adsorbent was characterized with scanning electron microscopy (SEM), nitrogen adsorption‐desorption isotherms, the mercury intrusion method, infrared spectroscopic analysis, and elementary analysis. The extent of chiral separation was tested with rac ‐tryptophan ( rac ‐Trp) and rac ‐phenylalanine ( rac ‐Phe) as solutes. The results showed that the absorbent exhibited a high surface area, large pore volume, and bimodal macromesoporous structures, enabling fast mass transfer and high separation efficiency. A fast adsorption rate, reaching equilibrium in less than 6 min, and a high degree of chiral separation, with the enantiomer excess ( e.e .) value reaching as high as 100% in the first 10 min, was observed in a small (5 cm in height, 0.46 cm in internal diameter) packed column that could be regenerated with a pH 5.0 HAc‐NaAc buffer. The results show that monodispersed silica spheres with a high BSA loading have great potential for applications in large‐scale chiral separation processes. Chirality, 2009. © 2008 Wiley‐Liss, Inc.

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