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High‐speed microchip electrophoresis method for the separation of ( R , S )‐naproxen
Author(s) -
Guihen Elizabeth,
Hogan AnnaMarie,
Glen Jeremy D.
Publication year - 2009
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20575
Subject(s) - capillary electrophoresis , chemistry , naproxen , enantiomer , chromatography , cyclodextrin , resolution (logic) , electrophoresis , naproxen sodium , analytical chemistry (journal) , acetonitrile , chirality (physics) , theoretical plate , buffer solution , stereochemistry , medicine , nambu–jona lasinio model , alternative medicine , chiral symmetry breaking , physics , pathology , quantum mechanics , artificial intelligence , computer science , quark
In this research, a capillary electrophoretic method for the fast enantiomeric resolution of ( R , S )‐naproxen was investigated. Method development involved variation of applied potential, buffer concentration, buffer pH, and cyclodextrin concentration. The optimum electrophoretic separation conditions were 110 mM sodium acetate run buffer (pH 6.0), 30 mM methyl‐β‐cyclodextrin, 20% (v/v) acetonitrile, 25°C. The total length of capillary was 48 cm, (50 μm I.D.) with ultra violet (UV) detection at 232 nm. Using these conditions, the number of theoretical plates was close to one million (896,000/m). The possibility of achieving a fast chiral separation of ( R , S )‐naproxen on a microchip of 2.5 cm in length was investigated. Complete enantiomeric resolution of naproxen was achieved in less than 1 min, on this microchip platform, with linear imaging UV detection. This system had the advantage of real‐time separation monitoring, so that enantiomeric resolution could be visually observed, and high‐speed chiral analysis was realized. The microchip electrophoresis (MCE) separation was compared with the capillary electrophoresis (CE) separation with regards to speed, efficiency, separation platform, and precision. This work highlights the potential of CE and MCE in future chiral separations. Chirality, 2009. © 2008 Wiley‐Liss, Inc.