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Synthesis, characterization, and DNA‐binding of chiral complexes Δ‐ and Λ‐[Ru(bpy) 2 (pyip)] 2+
Author(s) -
Shi Shuo,
Yao TianMing,
Geng XiaoTing,
Jiang LingFeng,
Liu Jie,
Yang QingYuan,
Ji LiangNian
Publication year - 2009
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20573
Subject(s) - chemistry , intercalation (chemistry) , dna , enantiomer , stereochemistry , crystallography , bipyridine , inorganic chemistry , crystal structure , biochemistry
New chiral Ru(II) complexes Δ and Λ‐[Ru(bpy) 2 (pyip)](PF 6 ) 2 [(bpy = 2,2′‐bipyridine; pyip = (2‐(1‐pyrenyl)‐1 H ‐imidazo[4,5‐ f ] [1,10]phenanthroline] were synthesized and characterized by elemental analysis, 1 H NMR, ESI‐MS, IR, and CD spectra. Their DNA‐binding properties were studied by means of UV–vis, emission spectra, CD spectra and viscosity measurements. A subtle but detectable difference was observed in the interaction of both enantiomer with CT‐DNA. Spectroscopy experiments indicated that each of these complexes could interact with the DNA. The DNA‐binding of the Δ‐enantiomer was stronger than that of Λ‐enantiomer. DNA‐viscosity experiments provided evidence that both Δ‐ and Λ‐[Ru(bpy) 2 (pyip)](PF 6 ) 2 bound to DNA by intercalation. At the same time, the DNA‐photocleavage properties of the complexes were investigated too. Under irradiation with UV light, Ru(II) complexes showed different efficiency of cleaving DNA. Chirality, 2009. © 2008 Wiley‐Liss, Inc.