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Microscale HPLC enables a new paradigm for commercialization of complex chiral stationary phases
Author(s) -
Welch Christopher J.,
Hyun Myung Ho,
Kubota Takateru,
Schafer Wes,
Bernardoni Frank,
Choi Hee Jung,
Wu Naijun,
Gong Xiaoyi,
Lipshutz Bruce
Publication year - 2008
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20548
Subject(s) - microscale chemistry , chemistry , chromatography , enantiomer , high performance liquid chromatography , chiral stationary phase , chirality (physics) , chiral column chromatography , stationary phase , analyte , fraction (chemistry) , organic chemistry , chiral symmetry breaking , mathematics education , mathematics , physics , quantum mechanics , nambu–jona lasinio model , quark
The small column size (0.3 mm i.d. × 15 cm) used in microscale HPLC contains only a small fraction (<1%) of the chromatographic packing material of a typical analytical HPLC column. Consequently, chromatographic stationary phases that are prohibitively expensive in conventional HPLC, owing either to synthetic complexity or costly starting materials, may become commercially viable in the microscale format. To illustrate this point, a previously described, synthetically complex, crown ether chiral stationary phase was prepared and evaluated in the microscale format, showing excellent separation of the enantiomers of underivatized amine analytes. Chirality 2008. © 2008 Wiley‐Liss, Inc.