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Functionalization of magnetic nanoparticles with organic molecules: Loading level determination and evaluation of linker length effect on immobilization
Author(s) -
Srinivasan Balasubramanian,
Huang Xuefei
Publication year - 2008
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20418
Subject(s) - silanization , chemistry , surface modification , linker , nanoparticle , rhodamine , magnetic nanoparticles , rhodamine b , molecule , bioconjugation , combinatorial chemistry , photochemistry , organic chemistry , nanotechnology , fluorescence , materials science , catalysis , physics , quantum mechanics , photocatalysis , computer science , operating system
A general method is introduced to immobilize organic molecules on magnetic nanoparticles through silanization reactions and determine the maximum loading level by UV–vis spectroscopy. Loading levels of 1.5 × 10 −3 mol per gram of nanoparticle were obtained with structurally diverse compounds such as rhodamine B and glucosamine. The length of the linker did not have a significant effect on loading as comparable maximum amounts of rhodamine B were immobilized on magnetic nanoparticles regardless of the linker length. Interestingly, rhodamine B derivatives lost conjugation during synthetic manipulations due to reversible spiroisobenzofuran formation. Full regeneration of conjugation was found to be slow with rhodamine B attached on magnetic nanoparticles. The results obtained from these studies will be useful for studying surface functionalization of MNPs in general. Chirality, 2008. © 2007 Wiley‐Liss, Inc.