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Determination of bevantolol enantiomers in human plasma by coupled achiral–chiral high performance‐liquid chromatography
Author(s) -
Oh Joung Weon,
Trung Tran Quoc,
Sin Kwan Seog,
Kang Jong Seong,
Kim Kyeong Ho
Publication year - 2007
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20410
Subject(s) - chemistry , enantiomer , chromatography , high performance liquid chromatography , chirality (physics) , chiral column chromatography , analytical chemistry (journal) , stereochemistry , chiral symmetry breaking , physics , quantum mechanics , nambu–jona lasinio model , quark
A coupled achiral–chiral high performance liquid chromatographic method was developed and fully validated for the determination of bevantolol enantiomers, (−)‐( S )‐bevantolol and (+)‐( R )‐bevantolol, in human plasma. Plasma samples were prepared by solid phase extraction with Sep‐Pak Plus C18 cartridges followed by HPLC. Bevantolol enantiomers and (+)‐( R )‐Propranolol as internal standard (IS) were preseparated from interfering components in plasma on a Phenomenex silica column and bevantolol enantiomers and IS were resolved and determined on a Chiralcel OJ‐H chiral stationary phase. The two columns were connected by a switching valve equipped with silica precolumn. The Precolumn was used to concentrate bevantolol in the eluent from the achiral column before back flushing onto chiral phase. A detailed validation of the method was performed accordingly to FDA guidelines. For each enantiomer the assay was linear between 20 and 1600 ng/ml. The quantification limits of both bevantolol enantiomers were 20 ng/ml. The intraday variation was between 1.07 and 12.64% in relation to the measured concentration and the interday variation was 0.91 and 11.79%. The method has been applied to the determination of (−)‐( S )‐ and (+)‐( R )‐bevantolol in plasma from healthy volunteers dosed with racemic bevantolol hydrochloride. hydrochloride. Chirality, 2007. © 2007 Wiley‐Liss, Inc.