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Determination of enantiomeric excess for 2,3‐dihydroxy‐3‐phenylpropionate compounds by capillary electrophoresis using hydroxypropyl‐β‐cyclodextrin as chiral selector
Author(s) -
Zhao Yan,
Yang XingBin,
Wang QiaoFeng,
Nan PengJuan,
Jin Ying,
Zhang ShengYong
Publication year - 2007
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20393
Subject(s) - chemistry , capillary electrophoresis , enantiomer , cyclodextrin , chirality (physics) , chromatography , enantiomeric excess , methanol , electrophoresis , impurity , analytical chemistry (journal) , stereochemistry , organic chemistry , enantioselective synthesis , catalysis , chiral symmetry breaking , physics , quantum mechanics , nambu–jona lasinio model , quark
A new capillary zone electrophoresis (CZE) method was developed to separate three chiral 2,3‐dihydroxy‐3‐phenylpropionate enantiomers using neutral hydroxypropyl‐β‐CD (HP‐β‐CD) as chiral selector and borate as background electrolyte. The results showed that HP‐β‐CD exhibited good enantioselectivity and high resolution was achieved under the optimum condition of pH 10.3, 200 mM borate buffer containing 6% methanol and 50 mM HP‐β‐CD at 15 kV and 20°C within 16 min. The precision of the method was <0.9% for migration time and 4.5% for corrected peak area. In addition, the developed method was successfully applied to the determination of enantiomeric excess ( ee ) of synthetic 2,3‐dihydroxy‐3‐phenylpropionate samples. With this method, low as 0.2% impurity of the undesirable enantiomer in the presence of high amount of target enantiomer was determined. The results demonstrated that the proposed CZE method is a simple and useful technique and is applicable to ee assay of 2,3‐dihydroxy‐3‐phenylpropionate enantiomers. Chirality, 2007. © 2007 Wiley‐Liss, Inc.