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Multiparallel chiral method development screening using an 8‐channel microfluidic HPLC system
Author(s) -
Sajonz Peter,
Gong Xiaoyi,
Leonard William R.,
Biba Mirlinda,
Welch Christopher J.
Publication year - 2006
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20325
Subject(s) - chemistry , chirality (physics) , high performance liquid chromatography , microfluidics , chromatography , development (topology) , channel (broadcasting) , column (typography) , analytical chemistry (journal) , nanotechnology , computer science , materials science , physics , mathematical analysis , computer network , telecommunications , mathematics , frame (networking) , chiral symmetry breaking , quantum mechanics , nambu–jona lasinio model , quark
The Eksigent Express 800 8‐channel microfluidic HPLC system was investigated for carrying out multiparallel screening and development of fast normal phase chiral separations. In contrast to the familiar automated sequential chiral method development approaches that often afford a next day result, the multiparallel approach offers the exciting possibility of near “real time” method development, often affording an optimized method in less than 1 h. In this study, four column types (300 μm i.d.) with two different mobile phases are screened using a universal standard gradient approach. Interestingly, parallel method optimization following initial screening was shown to sometimes lead to surprising and unanticipated outcomes, emphasizing the value of the multiparallel screening approach. A variety of standard test racemates were analyzed, with optimized separation methods for most in the 1‐ to 2‐min range. These results compare favorably with results obtained on a single channel conventional HPLC system using 4.6‐mm i.d. columns. In addition, isocratic methods developed on the microbore columns are readily translated to the larger column format. Chirality, 2006. © 2006 Wiley‐Liss, Inc.