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Enantiomer separation of amino acids in immunoaffinity micro LC‐MS
Author(s) -
Zeleke Jessica M.,
Smith Gregory B.,
Hofstetter Heike,
Hofstetter Oliver
Publication year - 2006
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20286
Subject(s) - chemistry , enantiomer , amino acid , chirality (physics) , chromatography , analyte , chiral stationary phase , chiral column chromatography , stereochemistry , biochemistry , chiral symmetry breaking , physics , quantum mechanics , nambu–jona lasinio model , quark
Chiral immunoaffinity microbore columns were directly interfaced with MS detection, and the effect of column length and temperature on the enantiomer separation of a number of underivatized aromatic and aliphatic amino acids was investigated utilizing an antibody chiral stationary phase that had been prepared by immobilizing a monoclonal anti‐ D ‐amino acid antibody onto silica. The stronger affinity of the antibody towards aromatic and bulky amino acids allowed separation of such analytes in a 0.75 × 150 mm column, while an increase in column length enabled separation of more weakly bound compounds. The strength of interaction between chiral selector and analytes could be modulated conveniently by lowering the temperature. For the first time, simultaneous enantiomer separation of mixtures of amino acids was achieved on antibody‐based chiral stationary phases using extracted ion chromatograms. Chirality 18:544–550, 2006. © 2006 Wiley‐Liss, Inc.