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Enantiomeric separation of mineralocorticoid receptor (hMR) antagonists using the Chiralcel® OJ‐H HPLC column with novel polar cosolvent eluent systems
Author(s) -
Sharp V. Scott,
Kennedy Joseph H.,
Belvo Matthew D.,
Williams Jeffrey D.,
Risley Donald S.,
Seest Eric P.
Publication year - 2006
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20269
Subject(s) - chemistry , acetonitrile , chromatography , enantiomer , high performance liquid chromatography , column chromatography , alcohol , polar , hexane , resolution (logic) , organic chemistry , physics , astronomy , artificial intelligence , computer science
This study demonstrates the increased versatility of the Chiralcel® OJ‐H stationary phase when using various alcohol/acetonitrile mobile phases. This chiral stationary phase has traditionally been employed in the normal phase mode and more recently with neat alcohols as eluents. Selected isomeric human mineralocorticoid receptor (hMR) antagonist pharmaceutical candidates and synthetic intermediates were separated using the Chiralcel® OJ‐H HPLC column with novel polar cosolvent eluent systems. The capacity factors, resolution, and selectivity of the chiral separations were assessed while varying the alcohol/acetonitrile composition and alcohol identity. The mixed polar eluents provide separations that are nearly always superior to both the traditional hexane‐rich and single‐alcohol “polar organic” eluents for the compounds tested in this article. Chirality, 2006. © 2006 Wiley‐Liss, Inc.