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Chiral separations on polysaccharide stationary phases using polar organic mobile phases
Author(s) -
Lynam Kenneth G.,
Stringham Rodger W.
Publication year - 2005
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20212
Subject(s) - chemistry , acetonitrile , polar , chirality (physics) , solubility , hexane , titration , chromatography , organic chemistry , physics , chiral symmetry breaking , quantum mechanics , astronomy , nambu–jona lasinio model , quark
About 30% of a chemically diverse set of compounds were found to separate on four polysaccharide chiral stationary phases using polar organic mobile phases. No structural features appeared to correlate to successful separations. Titrations between normal and polar organic mobile phases suggested that separation mechanisms do not differ between these mobile phases. Attempts made to control retention met with varying degrees of success. Addition of hexane to alcohols had minor effects on retention although this was occasionally beneficial. Addition of water to alcohols increased retention. Addition of water to acetonitrile decreased retention. Addition of alcohol to acetonitrile also proved beneficial to the separation of some compounds. Loading studies performed to mimic preparative separations indicated that the benefits of polar organic mobile phases are largely due to increased solubility. © 2005 Wiley‐Liss, Inc. Chirality