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Time‐resolved fluorescent chirality sensing and imaging of malate in aqueous solution
Author(s) -
Lin Zhihong,
Wu Meng,
Wolfbeis Otto S.
Publication year - 2005
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.20185
Subject(s) - chemistry , fluorescence , chirality (physics) , enantiomer , europium , aqueous solution , ternary operation , ternary complex , fluorescence lifetime imaging microscopy , photochemistry , analytical chemistry (journal) , chromatography , stereochemistry , organic chemistry , optics , ion , chiral symmetry breaking , physics , enzyme , quantum mechanics , computer science , nambu–jona lasinio model , programming language , quark
Chiral discrimination of malates in aqueous solutions at near‐neutral pH is achieved through fluorescence measurement and imaging using the europium–tetracycline complex (EuTc) as a fluorescent probe. The method is based on the significantly different fluorescence properties of the ternary complexes (Eu–Tc–malate) formed between EuTc and the enantiomeric malates. The enantiomeric excess (ee) of chiral malates can be quantified by both steady‐state and time‐resolved fluorescence, using either a conventional fluorescence microplate reader or fluorescence imaging. It offers a facile and sensitive method for high‐throughput chiral discrimination. © 2005 Wiley‐Liss, Inc. Chirality 17:464–469, 2005.