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Separation of cefoperazone enantiomers using β‐cyclodextrin as chiral additive by capillary zone electrophoresis
Author(s) -
Wang Rong,
Jia ZhengPing,
Fan JunJie,
Hu XiaoLi,
Li YongMin,
Chen LiRen,
Xie JingWen,
Zhang Qiang
Publication year - 2003
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.10305
Subject(s) - chemistry , cyclodextrin , enantiomer , capillary electrophoresis , chromatography , detection limit , cefoperazone , capillary action , calibration curve , chirality (physics) , electrophoresis , buffer solution , stereochemistry , biochemistry , materials science , nambu–jona lasinio model , chiral symmetry breaking , physics , antibiotic resistance , imipenem , quantum mechanics , composite material , quark , antibiotics
A capillary electrophoresis method was developed to separate the enantiomers of cefoperazone. Different cyclodextrins, including α‐cyclodextrin (α‐CD), β‐cyclodextrin (β‐CD), γ‐cyclodextrin (γ‐CD), 2‐hydroxypropyl‐β‐cyclodextrin (HP‐β‐CD), and methyl‐β‐cyclodextrin (Me‐β‐CD), were tested as chiral additives in the running buffer. The effect of various parameters on enantioseparation such as concentration of NaH 2 PO 4 , buffer pH, and CD concentration was also studied. The cefoperazone enantiomers were baseline separated under conditions of 0.04 mmol/L β‐CD, 75 mmol/L NaH 2 PO 4 buffer at pH 4.0. A fused silica capillary (40 cm effective length × 75 μm ID) was used. The applied voltage and capillary temperature were 20 kV and 25°C, respectively. Under these conditions, linear calibration curves were obtained in the 5–500 μg/ml range using UV detection at 280 nm. The limit of detection for both isomers was 0.1 μg/ml. The method was used for the analysis of different pharmaceutical preparations (dose) and biological samples containing cefoperazone. Chirality 16:45–49, 2004. © 2003 Wiley‐Liss, Inc.