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Study on the sorption properties of α 1 ‐acid glycoprotein (AGP)‐based stationary phase modified by organic solvents
Author(s) -
GyimesiForrás Krisztina,
Szász György,
Báthory Gábor,
Mészáros György,
Gergely András
Publication year - 2003
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.10213
Subject(s) - chemistry , sorption , acetonitrile , enantiomer , phase (matter) , quenching (fluorescence) , organic chemistry , chromatography , selectivity , fluorescence , catalysis , physics , adsorption , quantum mechanics
An α 1 ‐acid glycoprotein, immobilized on silica (Chiral‐AGP) is one of the most widely used chiral stationary phases for the enantiomeric separation of a wide variety of chiral drugs with several applications in the biological and clinical field. The aim of this work was to study the sorption properties of the AGP‐based stationary phase, which may have crucial importance for enantioselectivity. New binding data to the mechanism of the chromatographic separation are presented. The sorption of both organic solvents, i.e., acetonitrile and dioxane, shows remarkable pH dependency. A fluorescence quenching study was carried out to elucidate structural changes of AGP in the presence of acetonitrile using 2,2,2‐trichloroethanol as fluorescence quencher. Chirality 15:377–381, 2003. © 2003 Wiley‐Liss, Inc.