Premium
Development of a nonchiral HPLC method with circular dichroism detection for chiral analysis of molybdenum‐catalyzed enantioselective synthesis products
Author(s) -
Miller Michael T.,
Ge Zhihong,
Mao Bing
Publication year - 2002
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.10119
Subject(s) - chemistry , enantiomer , circular dichroism , enantioselective synthesis , detection limit , tsuji–trost reaction , chiral ligand , molybdenum , enantiomeric excess , chiral column chromatography , chiral derivatizing agent , ligand (biochemistry) , catalysis , chirality (physics) , high performance liquid chromatography , chromatography , combinatorial chemistry , stereochemistry , organic chemistry , biochemistry , receptor , nambu–jona lasinio model , chiral symmetry breaking , physics , quantum mechanics , quark
The combination of a circular dichroism detector and nonchiral liquid chromatography was used for the chiral analysis of unresolved enantiomers to determine the enantioselectivity of a molybdenum‐catalyzed asymmetric allylic alkylation reaction. The CD/UV peak area ratio of the unresolved enantiomers was calculated and compared with that of a reference standard to determine the enantiomeric purity. The limit of quantitation (LOQ) is 20 ng for the chiral ligand and 1 μg for the branched chiral product. The viability of the system depends on the limit of quantitation of the CD response and the linearity range of the CD and UV response. Chirality 14:659–664, 2002. © 2002 Wiley‐Liss, Inc.