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Direct chiral separation of unnatural amino acids by high‐performance liquid chromatography on a ristocetin a‐bonded stationary phase
Author(s) -
Török Gabriella,
Pëter Antal,
Armstrong Daniel W.,
Tourwë Drik,
Töth Gëza,
Säpi Jänos
Publication year - 2001
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/chir.10004
Subject(s) - chemistry , chromatography , selectivity , phase (matter) , high performance liquid chromatography , enantiomer , resolution (logic) , polar , amino acid , chromatographic separation , chiral stationary phase , chiral derivatizing agent , analyte , diastereomer , chiral column chromatography , organic chemistry , catalysis , biochemistry , physics , astronomy , artificial intelligence , computer science
Direct high‐performance liquid chromatographic chiral separation of numerous underivatized unnatural amino acids on a ristocetin A‐bonded chiral stationary phase used in the reversed‐phase and in the polar organic chromatographic modes is reported. The effects of different parameters such as mobile phase composition, temperature, and the structure of the analytes on the selectivity in both chromatographic modes are discussed. By variation of the parameters, the separation of the stereoisomers was optimized and, as a result, baseline resolution was achieved in most cases. Chirality 13:648–656, 2001. © 2001 Wiley‐Liss, Inc.