Premium
On the Mediated Electron Transfer of Immobilized Galactose Oxidase for Biotechnological Applications
Author(s) -
Zhao Fangyuan,
Brix Ann Cathrin,
Lielpetere Anna,
Schuhmann Wolfgang,
Conzuelo Felipe
Publication year - 2022
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202200868
Subject(s) - galactose oxidase , chemistry , electron transfer , substrate (aquarium) , catalysis , glycerol , redox , biocatalysis , immobilized enzyme , enzyme catalysis , electrochemistry , combinatorial chemistry , glucose oxidase , organic chemistry , chemical engineering , enzyme , electrode , reaction mechanism , oceanography , engineering , geology
The use of enzymes as catalysts in chemical synthesis offers advantages in terms of clean and highly selective transformations. Galactose oxidase (GalOx) is a remarkable enzyme with several applications in industrial conversions as it catalyzes the oxidation of primary alcohols. We have investigated the wiring of GalOx with a redox polymer; this enables mediated electron transfer with the electrode surface for its potential application in biotechnological conversions. As a result of electrochemical regeneration of the catalytic center, the formation of harmful H 2 O 2 is minimized during enzymatic catalysis. The introduced bioelectrode was applied to the conversion of bio‐renewable platform materials, with glycerol as model substrate. The biocatalytic transformations of glycerol and 5‐hydroxymethylfurfural (HMF) were investigated in a circular flow‐through setup to assess the possibility of substrate over‐oxidation, which is observed for glycerol oxidation but not during HMF conversion.