Premium
Highly Efficient Trityl‐Nitroxide Biradicals for Biomolecular High‐Field Dynamic Nuclear Polarization
Author(s) -
Cai Xinyi.,
Lucini Paioni Alessandra,
Adler Agnes,
Yao Ru,
Zhang Wenxiao,
Beriashvili David,
Safeer Adil,
Gurinov Andrei,
Rockenbauer Antal,
Song Yuguang,
Baldus Marc,
Liu Yangping
Publication year - 2021
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202102253
Subject(s) - biomolecule , nitroxide mediated radical polymerization , spectroscopy , nuclear magnetic resonance spectroscopy , polarization (electrochemistry) , small molecule , materials science , chemistry , molecule , nanotechnology , nuclear magnetic resonance , organic chemistry , physics , polymer , biochemistry , radical polymerization , quantum mechanics , copolymer
Dynamic nuclear polarization (DNP) is a powerful method to enhance the sensitivity of solid‐state magnetic nuclear resonance (ssNMR) spectroscopy. However, its biomolecular applications at high magnetic fields (preferably>14 T) have so far been limited by the intrinsically low efficiency of polarizing agents and sample preparation aspects. Herein, we report a new class of trityl‐nitroxide biradicals, dubbed SNAPols that combine high DNP efficiency with greatly enhanced hydrophilicity. SNAPol‐1, the best compound in the series, shows DNP enhancement factors at 18.8 T of more than 100 in small molecules and globular proteins and also exhibits strong DNP enhancements in membrane proteins and cellular preparations. By integrating optimal sensitivity and high resolution, we expect widespread applications of this new polarizing agent in high‐field DNP/ssNMR spectroscopy, especially for complex biomolecules.