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Trifaceted Mickey Mouse Amphiphiles for Programmable Self‐Assembly, DNA Complexation and Organ‐Selective Gene Delivery
Author(s) -
CarbajoGordillo Ana I.,
GonzálezCuesta Manuel,
Jiménez Blanco José L.,
Benito Juan M.,
SantanaArmas María L.,
Carmona Thais,
Di Giorgio Christophe,
Przybylski Cédric,
Ortiz Mellet Carmen,
Tros de Ilarduya Conchita,
Mendicuti Francisco,
García Fernández José M.
Publication year - 2021
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202100832
Subject(s) - gene delivery , amphiphile , cationic polymerization , transfection , nanotechnology , dna , chemistry , biophysics , combinatorial chemistry , materials science , polymer , organic chemistry , gene , biochemistry , biology , copolymer
Instilling segregated cationic and lipophilic domains with an angular disposition in a trehalose‐based trifaceted macrocyclic scaffold allows engineering patchy molecular nanoparticles leveraging directional interactions that emulate those controlling self‐assembling processes in viral capsids. The resulting trilobular amphiphilic derivatives, featuring a Mickey Mouse architecture, can electrostatically interact with plasmid DNA (pDNA) and further engage in hydrophobic contacts to promote condensation into transfectious nanocomplexes. Notably, the topology and internal structure of the cyclooligosaccharide/pDNA co‐assemblies can be molded by fine‐tuning the valency and characteristics of the cationic and lipophilic patches, which strongly impacts the transfection efficacy in vitro and in vivo. Outstanding organ selectivities can then be programmed with no need of incorporating a biorecognizable motif in the formulation. The results provide a versatile strategy for the construction of fully synthetic and perfectly monodisperse nonviral gene delivery systems uniquely suited for optimization schemes by making cyclooligosaccharide patchiness the focus.